Use of free and immobilized Pseudomonas putida cells for the reduction of a thiophene derivative in organic media

P. Fernandes, P. Vidinha, T. Ferreira, H. Silvestre, J. M.S. Cabral, D. M.F. Prazeres

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26 Citações (Scopus)

Resumo

An organic-aqueous two-liquid phase system was developed in order to perform the reduction of 3-thiophenecarboxaldeyde (3-TC) to 3-thiophenemethanol (3-TM), two intermediate logP compounds, using Pseudomonas putida S12 cells. Although, high bioconversion yields were observed in a bioconversion medium composed by Tris-HCl pH 8.5 buffer and n-octanol, adverse phase toxicity effects were observed following incubation periods in excess of 24h. Furthermore, a stable emulsion was formed in two-liquid phase system in the presence of free cells. This prevented effective separation of the biocatalyst and of the two-liquid phases. Whole cells were effectively entrapped in Ca-alginate beads, thus enhancing biocatalyst recovery. No shifts in the pH versus activity profile resulted from the immobilization. Successive batches were carried out with the immobilized biocatalyst, but a decrease in the specific activity was observed.

Idioma originalInglês
Páginas (de-até)353-361
Número de páginas9
RevistaJournal of Molecular Catalysis - B Enzymatic
Volume19-20
DOIs
Estado da publicaçãoPublicadas - 2 dez. 2002

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