Study of key operational parameters for the side-chain cleavage of sitosterol by free mycobacterial cells in bis-(2-ethylhexyl) phthalate

A. Cruz, B. Angelova, P. Fernandes, J. M.S. Cabral, Helena M. Pinheiro

Resultado de pesquisarevisão de pares

19 Citações (Scopus)

Resumo

The selective side-chain cleavage of β-sitosterol by free cells of Mycobacterium sp. NRRL B-3805 is a well-established multi-enzymatic process for the production of the pharmaceutical steroid precursors androstenedione (AD) and androstadienedione (ADD). In this study, bis(2-ethylhexyl) phthalate (BEHP) was used as a reaction medium for carrying out the process with freely suspended cells. The work aimed to show that microbial sitosterol side-chain cleavage is possible in this essentially mono-phasic organic medium, provided that some important parameters are adequately controlled. The effects of the biocatalyst/substrate mass ratio, system aeration rate and minimum buffer addition to the organic medium on the product yield and the reaction rate were thus evaluated.

Idioma originalInglês
Páginas (de-até)189-194
Número de páginas6
RevistaBiocatalysis and Biotransformation
Volume22
Número de emissão3
DOIs
Estado da publicaçãoPublicadas - mai. 2004

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