Resumo
The selective side-chain cleavage of β-sitosterol by free cells of Mycobacterium sp. NRRL B-3805 is a well-established multi-enzymatic process for the production of the pharmaceutical steroid precursors androstenedione (AD) and androstadienedione (ADD). In this study, bis(2-ethylhexyl) phthalate (BEHP) was used as a reaction medium for carrying out the process with freely suspended cells. The work aimed to show that microbial sitosterol side-chain cleavage is possible in this essentially mono-phasic organic medium, provided that some important parameters are adequately controlled. The effects of the biocatalyst/substrate mass ratio, system aeration rate and minimum buffer addition to the organic medium on the product yield and the reaction rate were thus evaluated.
Idioma original | Inglês |
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Páginas (de-até) | 189-194 |
Número de páginas | 6 |
Revista | Biocatalysis and Biotransformation |
Volume | 22 |
Número de emissão | 3 |
DOIs | |
Estado da publicação | Publicadas - mai. 2004 |