TY - JOUR
T1 - Immobilization of Fungal Cellulases Highlighting β-Glucosidase
T2 - Techniques, Supports, Chemical, and Physical Changes
AU - da Silva Almeida, Larissa Emanuelle
AU - Fernandes, Pedro
AU - de Assis, Sandra Aparecida
N1 - Publisher Copyright:
© 2022, The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.
PY - 2022/4
Y1 - 2022/4
N2 - β-Glucosidase is widely used in several industrial segments, among which we can highlight the pharmaceutical industry, beverages, biofuels, animal feed production, and the textile industry. The great applicability of this enzyme, associated with the high cost of its production, justifies the need to find ways to make its use economically viable on an industrial scale. Through enzyme immobilization, the biocatalyst can be reused more than once, without great impact on its catalytic activity, and higher operational and storage stabilities can be achieved as compared to the free form. Accordingly, this review brings information about different techniques and supports that have been studied in the immobilization of cellulases with a focus on β-glucosidase, as well as the application of these immobilized systems to supplement commercial mixtures.
AB - β-Glucosidase is widely used in several industrial segments, among which we can highlight the pharmaceutical industry, beverages, biofuels, animal feed production, and the textile industry. The great applicability of this enzyme, associated with the high cost of its production, justifies the need to find ways to make its use economically viable on an industrial scale. Through enzyme immobilization, the biocatalyst can be reused more than once, without great impact on its catalytic activity, and higher operational and storage stabilities can be achieved as compared to the free form. Accordingly, this review brings information about different techniques and supports that have been studied in the immobilization of cellulases with a focus on β-glucosidase, as well as the application of these immobilized systems to supplement commercial mixtures.
KW - Adsorption
KW - Covalent bond
KW - Entrapment
KW - β-Glucosidase
UR - http://www.scopus.com/inward/record.url?scp=85128413167&partnerID=8YFLogxK
U2 - 10.1007/s10930-022-10048-7
DO - 10.1007/s10930-022-10048-7
M3 - Review article
C2 - 35438380
AN - SCOPUS:85128413167
SN - 1572-3887
VL - 41
SP - 274
EP - 292
JO - Protein Journal
JF - Protein Journal
IS - 2
ER -