Resumo
The use of organic solvent-based media for microbial 9α-hydroxylation of androstenedione (AD) by resting Rhodococcus sp. cells was evaluated. Product yields higher than 90% were observed in a two-phase phthalate-buffer transformation medium (3:1, v/v) containing 1.0 g L-1 AD. The results suggest that in these conditions the reaction of Δ1- dehydrogenation is considerably inhibited. The successful hydroxylation reaction with a product yield exceeding 60% was found to take place also in a phthalate medium under simultaneous forced aeration and maintenance of a minimum water content of about 3% (v/v).
Idioma original | Inglês |
---|---|
Páginas (de-até) | 718-722 |
Número de páginas | 5 |
Revista | Enzyme and Microbial Technology |
Volume | 37 |
Número de emissão | 7 |
DOIs | |
Estado da publicação | Publicadas - 1 dez. 2005 |