Hydroxylation of androstenedione by resting Rhodococcus sp. cells in organic media

B. Angelova, P. Fernandes, A. Cruz, H. M. Pinheiro, S. Mutafov, J. M.S. Cabral

Resultado de pesquisarevisão de pares

19 Citações (Scopus)

Resumo

The use of organic solvent-based media for microbial 9α-hydroxylation of androstenedione (AD) by resting Rhodococcus sp. cells was evaluated. Product yields higher than 90% were observed in a two-phase phthalate-buffer transformation medium (3:1, v/v) containing 1.0 g L-1 AD. The results suggest that in these conditions the reaction of Δ1- dehydrogenation is considerably inhibited. The successful hydroxylation reaction with a product yield exceeding 60% was found to take place also in a phthalate medium under simultaneous forced aeration and maintenance of a minimum water content of about 3% (v/v).

Idioma originalInglês
Páginas (de-até)718-722
Número de páginas5
RevistaEnzyme and Microbial Technology
Volume37
Número de emissão7
DOIs
Estado da publicaçãoPublicadas - 1 dez. 2005

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