Enzyme Immobilization and Co-Immobilization: Main Framework, Advances and Some Applications

Joaquim Bié, Bruno Sepodes, Pedro C.B. Fernandes, Maria H.L. Ribeiro

Resultado de pesquisarevisão de pares

85 Citações (Scopus)

Resumo

Enzymes are outstanding (bio)catalysts, not solely on account of their ability to increase reaction rates by up to several orders of magnitude but also for the high degree of substrate specificity, regiospecificity and stereospecificity. The use and development of enzymes as robust biocatalysts is one of the main challenges in biotechnology. However, despite the high specificities and turnover of enzymes, there are also drawbacks. At the industrial level, these drawbacks are typically overcome by resorting to immobilized enzymes to enhance stability. Immobilization of biocatalysts allows their reuse, increases stability, facilitates process control, eases product recovery, and enhances product yield and quality. This is especially important for expensive enzymes, for those obtained in low fermentation yield and with relatively low activity. This review provides an integrated perspective on (multi)enzyme immobilization that abridges a critical evaluation of immobilization methods and carriers, biocatalyst metrics, impact of key carrier features on biocatalyst performance, trends towards miniaturization and detailed illustrative examples that are representative of biocatalytic applications promoting sustainability.

Idioma originalInglês
Número do artigo494
RevistaProcesses
Volume10
Número de emissão3
DOIs
Estado da publicaçãoPublicadas - mar. 2022

Nota bibliográfica

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© 2022 by the authors. Licensee MDPI, Basel, Switzerland.

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