Enlarged PLIN5-uncoated lipid droplets in inner regions of skeletal muscle type II fibers associate with type 2 diabetes

Vasco Fachada; Paavo Rahkila; Nuno Fachada; Tuomas Turpeinen; Urho M. Kujala; Heikki Kainulainen

doi:10.1016/j.acthis.2022.151869

Enlarged PLIN5-uncoated lipid droplets in inner regions of skeletal muscle type II fibers associate with type 2 diabetes

Vasco Fachada, Paavo Rahkila, Nuno Fachada, Tuomas Turpeinen, Urho M. Kujala, Heikki Kainulainen

COPELABS - Centro de Investigação em Computação Centrada nas Pessoas e Cognição

University of Jyväskylä

Resultado de pesquisa › revisão de pares

4 Citações (Scopus)

Resumo

Skeletal muscle physiology remains of paramount importance in understanding insulin resistance. Due to its high lipid turnover rates, regulation of intramyocellular lipid droplets (LDs) is a key factor. Perilipin 5 (PLIN5) is one of the most critical agents in such regulation, being often referred as a protector against lipotoxicity and consequent skeletal muscle insulin resistance. We examined area fraction, size, subcellular localization and PLIN5 association of LDs in two fiber types of type 2 diabetic (T2D), obese (OB) and healthy (HC) individuals by means of fluorescence microscopy and image analysis. We found that T2D type II fibers have a significant sub-population of large and internalized LDs, uncoated by PLIN5. Based on this novel result, additional hypotheses for the pathophysiology of skeletal muscle insulin resistance are formulated, together with future research directions.

Idioma original	Inglês
Número do artigo	151869
Revista	Acta Histochemica
Volume	124
Número de emissão	3
DOIs	https://doi.org/10.1016/j.acthis.2022.151869
Estado da publicação	Publicadas - abr. 2022

Financiamento

This study was supported by the Academy of Finland (grant 132987 and 298875) and Fundaç˜ ao para a Ciˆ encia e a Tecnologia (FCT, Portugal) under grant UIDB/04111/2020 (COPELABS). The author Vasco Fachada acknowledges his FCT grant SFRH/BD/68308/2010

Keywords

BIOLOGIA
LÍPIDOS
DIABETES TIPO 2
MÚSCULOS ESQUELÉTICOS
RESISTÊNCIA À INSULINA

ODS da ONU

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title = "Enlarged PLIN5-uncoated lipid droplets in inner regions of skeletal muscle type II fibers associate with type 2 diabetes",

abstract = "Skeletal muscle physiology remains of paramount importance in understanding insulin resistance. Due to its high lipid turnover rates, regulation of intramyocellular lipid droplets (LDs) is a key factor. Perilipin 5 (PLIN5) is one of the most critical agents in such regulation, being often referred as a protector against lipotoxicity and consequent skeletal muscle insulin resistance. We examined area fraction, size, subcellular localization and PLIN5 association of LDs in two fiber types of type 2 diabetic (T2D), obese (OB) and healthy (HC) individuals by means of fluorescence microscopy and image analysis. We found that T2D type II fibers have a significant sub-population of large and internalized LDs, uncoated by PLIN5. Based on this novel result, additional hypotheses for the pathophysiology of skeletal muscle insulin resistance are formulated, together with future research directions.",

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author = "Vasco Fachada and Paavo Rahkila and Nuno Fachada and Tuomas Turpeinen and Kujala, {Urho M.} and Heikki Kainulainen",

year = "2022",

month = apr,

doi = "10.1016/j.acthis.2022.151869",

language = "English",

volume = "124",

journal = "Acta Histochemica",

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TY - JOUR

T1 - Enlarged PLIN5-uncoated lipid droplets in inner regions of skeletal muscle type II fibers associate with type 2 diabetes

AU - Fachada, Vasco

AU - Rahkila, Paavo

AU - Fachada, Nuno

AU - Turpeinen, Tuomas

AU - Kujala, Urho M.

AU - Kainulainen, Heikki

PY - 2022/4

Y1 - 2022/4

N2 - Skeletal muscle physiology remains of paramount importance in understanding insulin resistance. Due to its high lipid turnover rates, regulation of intramyocellular lipid droplets (LDs) is a key factor. Perilipin 5 (PLIN5) is one of the most critical agents in such regulation, being often referred as a protector against lipotoxicity and consequent skeletal muscle insulin resistance. We examined area fraction, size, subcellular localization and PLIN5 association of LDs in two fiber types of type 2 diabetic (T2D), obese (OB) and healthy (HC) individuals by means of fluorescence microscopy and image analysis. We found that T2D type II fibers have a significant sub-population of large and internalized LDs, uncoated by PLIN5. Based on this novel result, additional hypotheses for the pathophysiology of skeletal muscle insulin resistance are formulated, together with future research directions.

AB - Skeletal muscle physiology remains of paramount importance in understanding insulin resistance. Due to its high lipid turnover rates, regulation of intramyocellular lipid droplets (LDs) is a key factor. Perilipin 5 (PLIN5) is one of the most critical agents in such regulation, being often referred as a protector against lipotoxicity and consequent skeletal muscle insulin resistance. We examined area fraction, size, subcellular localization and PLIN5 association of LDs in two fiber types of type 2 diabetic (T2D), obese (OB) and healthy (HC) individuals by means of fluorescence microscopy and image analysis. We found that T2D type II fibers have a significant sub-population of large and internalized LDs, uncoated by PLIN5. Based on this novel result, additional hypotheses for the pathophysiology of skeletal muscle insulin resistance are formulated, together with future research directions.

KW - BIOLOGIA

KW - LÍPIDOS

KW - DIABETES TIPO 2

KW - MÚSCULOS ESQUELÉTICOS

KW - RESISTÊNCIA À INSULINA

KW - BIOLOGY

KW - LIPIDS

KW - TYPE 2 DIABETES

KW - SKELETAL MUSCLE

KW - INSULIN RESISTANCE

UR - http://hdl.handle.net/10437/12738

UR - http://www.scopus.com/inward/record.url?scp=85125120283&partnerID=8YFLogxK

U2 - 10.1016/j.acthis.2022.151869

DO - 10.1016/j.acthis.2022.151869

M3 - Article

C2 - 35220055

SN - 0065-1281

VL - 124

JO - Acta Histochemica

JF - Acta Histochemica

IS - 3

M1 - 151869

ER -

Enlarged PLIN5-uncoated lipid droplets in inner regions of skeletal muscle type II fibers associate with type 2 diabetes

Resumo

Financiamento

Keywords

ODS da ONU

Acesso ao documento

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