β-Sitosterol Bioconversion in Small-Scale Devices: From Microtiter Plates to Microfluidic Reactors

Marco P.C. Marques, Jorge Aranda Hernandez, Pedro Fernandes

Resultado de pesquisarevisão de pares

Resumo

Small-scale devices are routinely used as low-cost miniaturized bioreactors due to the large number of experiments that can be conducted simultaneously under similar conditions and replicate all functions of bench-scale reactors at dramatically smaller volumes. Microtiter plates, due to the standard footprint, can be integrated with liquid handling systems and associated equipment, expanding considerably their application and use. However, care has to be taken to operate the microtiter plates in optimized mixing and oxygen transfer conditions, preventing medium evaporation in prolonged experiment runs. Recently, to increase data quality, microbioreactors have emerged as an alternative to shaken systems. These systems offer higher degree of control over key process variables and when combined with sensing technology increase dramatically the reliability of translational process data. In this chapter, we describe the production of 4-androstene-3,17-dione (androstenedione (AD)), a key pharmaceutical steroid intermediate, by Mycobacterium sp. NRRL B-3805 via the selective cleavage of the side-chain of β-sitosterol using 24-well microtiter plates and microfluidic microbioreactors.

Idioma originalInglês
Título da publicação do anfitriãoMethods in Molecular Biology
EditoraHumana Press Inc.
Páginas201-219
Número de páginas19
DOIs
Estado da publicaçãoPublicadas - 2023

Série de publicação

NomeMethods in Molecular Biology
Volume2704
ISSN (impresso)1064-3745
ISSN (eletrónico)1940-6029

Nota bibliográfica

Publisher Copyright:
© 2023, The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.

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