TY - JOUR
T1 - Screening of supports for the immobilization of pectinmethylesterase from acerola (Malpighia glabra L)
AU - Aparecida de Assis, Sandra
AU - Fernandes, Pedro
AU - Ferreira, Bruno Sommer
AU - Cabral, Joaquim M.S.
AU - Mascarenhas Faria Oliveira, Olga Maria
PY - 2004/3
Y1 - 2004/3
N2 - A partially purified extract of pectinmethylesterase (PME) from acerola fruit was immobilized on various supports: glass, celite, chrysotile, agarose, concanavalin A Sepharose 4B, egg shell, polyacrylamide and gelatin. In addition, reticulation with glutaraldehyde was assessed, as well as the use of gelatin in the presence of celite, glass and silica. The highest immobilization yields were obtained when the pectinmethylesterase was immobilized in concanavalin A Sepharose 4B (81.7%) and in gelatin-water (78.0%).
AB - A partially purified extract of pectinmethylesterase (PME) from acerola fruit was immobilized on various supports: glass, celite, chrysotile, agarose, concanavalin A Sepharose 4B, egg shell, polyacrylamide and gelatin. In addition, reticulation with glutaraldehyde was assessed, as well as the use of gelatin in the presence of celite, glass and silica. The highest immobilization yields were obtained when the pectinmethylesterase was immobilized in concanavalin A Sepharose 4B (81.7%) and in gelatin-water (78.0%).
KW - Acerola
KW - Immobilization
KW - Kinetics characterization
KW - Pectinmethylesterase
UR - http://www.scopus.com/inward/record.url?scp=1442277732&partnerID=8YFLogxK
U2 - 10.1002/jctb.973
DO - 10.1002/jctb.973
M3 - Article
AN - SCOPUS:1442277732
SN - 0268-2575
VL - 79
SP - 277
EP - 280
JO - Journal of Chemical Technology and Biotechnology
JF - Journal of Chemical Technology and Biotechnology
IS - 3
ER -