Immobilization of mycobacterial cells onto silicone - Assessing the feasibility of the immobilized biocatalyst in the production of androstenedione from sitosterol

M. J.C. Claudino; D. Soares; F. Van Keulen; M. P.C. Marques; J. M.S. Cabral; P. Fernandes

doi:10.1016/j.biortech.2007.05.007

Immobilization of mycobacterial cells onto silicone - Assessing the feasibility of the immobilized biocatalyst in the production of androstenedione from sitosterol

M. J.C. Claudino, D. Soares, F. Van Keulen, M. P.C. Marques, J. M.S. Cabral, P. Fernandes

Research output: Contribution to journal › Article › peer-review

22 Citations (Scopus)

Abstract

Silicone rubbers are hydrophobic, a feature that may prove advantageous if this material is to be used as immobilization matrix in bioconversion systems where hydrophobic species are present, such as sterols and mycobacterial cells. Mycobacterium sp. cells with sitosterol side chain cleavage activity were accordingly effectively adsorbed onto silicone and the potential application of the concept was assessed by matching the behavior of the resulting immobilized biocatalyst with free cells and Celite immobilized cells. Mass transfer, kinetics, thermal and storage stability characterization of a biotransformation system based in the use of the silicone immobilized biocatalyst was performed. The feasibility of biocatalyst reutilization was tentatively explored.

Original language	English
Pages (from-to)	2304-2311
Number of pages	8
Journal	Bioresource Technology
Volume	99
Issue number	7
DOIs	https://doi.org/10.1016/j.biortech.2007.05.007
Publication status	Published - May 2008

Funding

P. Fernandes and M.P.C. Marques acknowledge Grants SFRH/BPD/20416/2004 and SFRH/BD/24433/2005, respectively, from Fundação para a Ciência e a Tecnologia, Portugal. This work was partially funded by the research Project POCI/SAU-MMO/59370/2004 from Fundação para a Ciência e a Tecnologia (Portugal).

Funders	Funder number
Fundação para a Ciência e a Tecnologia	POCI/SAU-MMO/59370/2004

Keywords

4-Androstene-3,17-dione
Cell immobilization
Mycobacterium sp.
Silicone slabs
β-Sitosterol

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10.1016/j.biortech.2007.05.007

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abstract = "Silicone rubbers are hydrophobic, a feature that may prove advantageous if this material is to be used as immobilization matrix in bioconversion systems where hydrophobic species are present, such as sterols and mycobacterial cells. Mycobacterium sp. cells with sitosterol side chain cleavage activity were accordingly effectively adsorbed onto silicone and the potential application of the concept was assessed by matching the behavior of the resulting immobilized biocatalyst with free cells and Celite immobilized cells. Mass transfer, kinetics, thermal and storage stability characterization of a biotransformation system based in the use of the silicone immobilized biocatalyst was performed. The feasibility of biocatalyst reutilization was tentatively explored.",

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AU - Soares, D.

AU - Van Keulen, F.

AU - Marques, M. P.C.

AU - Cabral, J. M.S.

AU - Fernandes, P.

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Immobilization of mycobacterial cells onto silicone - Assessing the feasibility of the immobilized biocatalyst in the production of androstenedione from sitosterol

Abstract

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Keywords

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