Immobilization of Fungal Cellulases Highlighting β-Glucosidase: Techniques, Supports, Chemical, and Physical Changes

Larissa Emanuelle da Silva Almeida; Pedro Fernandes; Sandra Aparecida de Assis

doi:10.1007/s10930-022-10048-7

Immobilization of Fungal Cellulases Highlighting β-Glucosidase: Techniques, Supports, Chemical, and Physical Changes

Larissa Emanuelle da Silva Almeida
, Pedro Fernandes
, Sandra Aparecida de Assis

HEI-LAB - Human Environment Interaction Lab

Universidade Estadual de Feira de Santana
University of Lisbon

Research output: Contribution to journal › Review article › peer-review

8 Citations (Scopus)

Abstract

β-Glucosidase is widely used in several industrial segments, among which we can highlight the pharmaceutical industry, beverages, biofuels, animal feed production, and the textile industry. The great applicability of this enzyme, associated with the high cost of its production, justifies the need to find ways to make its use economically viable on an industrial scale. Through enzyme immobilization, the biocatalyst can be reused more than once, without great impact on its catalytic activity, and higher operational and storage stabilities can be achieved as compared to the free form. Accordingly, this review brings information about different techniques and supports that have been studied in the immobilization of cellulases with a focus on β-glucosidase, as well as the application of these immobilized systems to supplement commercial mixtures.

Original language	English
Pages (from-to)	274-292
Number of pages	19
Journal	Protein Journal
Volume	41
Issue number	2
DOIs	https://doi.org/10.1007/s10930-022-10048-7
Publication status	Published - Apr 2022

Bibliographical note

Publisher Copyright:
© 2022, The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.

Funding

We thank the Biotechnology Graduate Program of State University of Feira de Santana (UEFS/FIOCRUZ). This work was supported by one doctoral scholarship (88882.447813/2019-01) financed by CAPES (Coordination for the Improvement of Higher Education Personnel), FAPESB (Bahia State Research Support Foundation), and CNPq (National Council for Scientific and Technological Development).

Funders
State University of Feira de Santana
UEFS
Coordenação de Aperfeiçoamento de Pessoal de Nível Superior
Conselho Nacional de Desenvolvimento Científico e Tecnológico
Fundação de Amparo à Pesquisa do Estado da Bahia
Fundação Oswaldo Cruz

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 7 Affordable and Clean Energy

Keywords

Adsorption
Covalent bond
Entrapment
β-Glucosidase

Access to Document

10.1007/s10930-022-10048-7

Cite this

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title = "Immobilization of Fungal Cellulases Highlighting β-Glucosidase: Techniques, Supports, Chemical, and Physical Changes",

abstract = "β-Glucosidase is widely used in several industrial segments, among which we can highlight the pharmaceutical industry, beverages, biofuels, animal feed production, and the textile industry. The great applicability of this enzyme, associated with the high cost of its production, justifies the need to find ways to make its use economically viable on an industrial scale. Through enzyme immobilization, the biocatalyst can be reused more than once, without great impact on its catalytic activity, and higher operational and storage stabilities can be achieved as compared to the free form. Accordingly, this review brings information about different techniques and supports that have been studied in the immobilization of cellulases with a focus on β-glucosidase, as well as the application of these immobilized systems to supplement commercial mixtures.",

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doi = "10.1007/s10930-022-10048-7",

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T2 - Techniques, Supports, Chemical, and Physical Changes

AU - da Silva Almeida, Larissa Emanuelle

AU - Fernandes, Pedro

AU - de Assis, Sandra Aparecida

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AB - β-Glucosidase is widely used in several industrial segments, among which we can highlight the pharmaceutical industry, beverages, biofuels, animal feed production, and the textile industry. The great applicability of this enzyme, associated with the high cost of its production, justifies the need to find ways to make its use economically viable on an industrial scale. Through enzyme immobilization, the biocatalyst can be reused more than once, without great impact on its catalytic activity, and higher operational and storage stabilities can be achieved as compared to the free form. Accordingly, this review brings information about different techniques and supports that have been studied in the immobilization of cellulases with a focus on β-glucosidase, as well as the application of these immobilized systems to supplement commercial mixtures.

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KW - Covalent bond

KW - Entrapment

KW - β-Glucosidase

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DO - 10.1007/s10930-022-10048-7

M3 - Review article

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AN - SCOPUS:85128413167

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IS - 2

ER -

Immobilization of Fungal Cellulases Highlighting β-Glucosidase: Techniques, Supports, Chemical, and Physical Changes

Abstract

Bibliographical note

Funding

UN SDGs

Keywords

Access to Document

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Cite this