Hydroxylation of androstenedione by resting Rhodococcus sp. cells in organic media

B. Angelova; P. Fernandes; A. Cruz; H. M. Pinheiro; S. Mutafov; J. M.S. Cabral

doi:10.1016/j.enzmictec.2005.04.014

Hydroxylation of androstenedione by resting Rhodococcus sp. cells in organic media

B. Angelova, P. Fernandes, A. Cruz, H. M. Pinheiro, S. Mutafov, J. M.S. Cabral

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19 Citations (Scopus)

Abstract

The use of organic solvent-based media for microbial 9α-hydroxylation of androstenedione (AD) by resting Rhodococcus sp. cells was evaluated. Product yields higher than 90% were observed in a two-phase phthalate-buffer transformation medium (3:1, v/v) containing 1.0 g L^-1 AD. The results suggest that in these conditions the reaction of Δ¹- dehydrogenation is considerably inhibited. The successful hydroxylation reaction with a product yield exceeding 60% was found to take place also in a phthalate medium under simultaneous forced aeration and maintenance of a minimum water content of about 3% (v/v).

Original language	English
Pages (from-to)	718-722
Number of pages	5
Journal	Enzyme and Microbial Technology
Volume	37
Issue number	7
DOIs	https://doi.org/10.1016/j.enzmictec.2005.04.014
Publication status	Published - 1 Dec 2005

Funding

This study was supported by a NATO fellowship CP(BU)09/C/2001 awarded to B. Angelova by INVOTAN/ICCTI, Portugal and by a PhD grant, PRAXISXXI/BD/21393/99, awarded to A. Cruz by Fundação para a Ciência e a Tecnologia, Portugal.

Funders	Funder number
ICCTI	PRAXISXXI/BD/21393/99
INVOTAN
North Atlantic Treaty Organization	09/C/2001
Fundação para a Ciência e a Tecnologia

Keywords

Bis-(2-ethylhexyl) phthalate
Microbial transformation
Rhodococcus
Steroids

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10.1016/j.enzmictec.2005.04.014

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title = "Hydroxylation of androstenedione by resting Rhodococcus sp. cells in organic media",

abstract = "The use of organic solvent-based media for microbial 9α-hydroxylation of androstenedione (AD) by resting Rhodococcus sp. cells was evaluated. Product yields higher than 90% were observed in a two-phase phthalate-buffer transformation medium (3:1, v/v) containing 1.0 g L-1 AD. The results suggest that in these conditions the reaction of Δ1- dehydrogenation is considerably inhibited. The successful hydroxylation reaction with a product yield exceeding 60% was found to take place also in a phthalate medium under simultaneous forced aeration and maintenance of a minimum water content of about 3% (v/v).",

keywords = "Bis-(2-ethylhexyl) phthalate, Microbial transformation, Rhodococcus, Steroids",

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AU - Angelova, B.

AU - Fernandes, P.

AU - Cruz, A.

AU - Pinheiro, H. M.

AU - Mutafov, S.

AU - Cabral, J. M.S.

PY - 2005/12/1

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AB - The use of organic solvent-based media for microbial 9α-hydroxylation of androstenedione (AD) by resting Rhodococcus sp. cells was evaluated. Product yields higher than 90% were observed in a two-phase phthalate-buffer transformation medium (3:1, v/v) containing 1.0 g L-1 AD. The results suggest that in these conditions the reaction of Δ1- dehydrogenation is considerably inhibited. The successful hydroxylation reaction with a product yield exceeding 60% was found to take place also in a phthalate medium under simultaneous forced aeration and maintenance of a minimum water content of about 3% (v/v).

KW - Bis-(2-ethylhexyl) phthalate

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KW - Rhodococcus

KW - Steroids

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Hydroxylation of androstenedione by resting Rhodococcus sp. cells in organic media

Abstract

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