Evaluation of hematology, general serum biochemistry, bone turnover markers and bone marrow cytology in a glucocorticoid treated ovariectomized sheep model for osteoporosis research

Catarina A. Coelho; João P. Bordelo; José A. Camassa; Vera A. Barros; Pedro S. Babo; Manuela E. Gomes; Rui L. Reis; Jorge T. DE AZEVEDO; João F. Requicha; Pedro Faísca; Pedro P. Carvalho; Carlos A. Viegas; Isabel R. Dias

doi:10.1590/0001-3765202020200435

Evaluation of hematology, general serum biochemistry, bone turnover markers and bone marrow cytology in a glucocorticoid treated ovariectomized sheep model for osteoporosis research

Catarina A. Coelho, João P. Bordelo, José A. Camassa, Vera A. Barros, Pedro S. Babo, Manuela E. Gomes, Rui L. Reis, Jorge T. DE AZEVEDO, João F. Requicha, Pedro Faísca, Pedro P. Carvalho, Carlos A. Viegas, Isabel R. Dias

Research output: Contribution to journal › Review article › peer-review

3 Citations (Scopus)

Abstract

Osteoporosis is a metabolic disorder characterized by a loss of bone mass and structure and increasing the risk of fragility fractures, mostly among postmenopausal women. Sheep is a recognized large animal model for osteoporosis research. An experimental group of ewes (3-4 years old) was subjected to ovariectomy (OVX) and weekly glucocorticoid (GC) application for 24 weeks and compared with a sham control group. Blood and bone marrow parameters were analyzed before and 24 weeks after OVX and GC administration. Osteopenia was confirmed through micro-computed tomography and histomorphometric analysis of L4 vertebra in the study end. A statistically significant increase was observed in mean corpuscular volume, mean cell hemoglobin and monocytes and a decrease in red blood count and eosinophils (p<0.05). Alkaline phosphatase (ALP), gamma-glutamyl transpeptidase, magnesium and α1-globulin increased, and creatinine, albumin, sodium and estradiol decreased (p<0.05). A slight decrease of bone formation markers (bone ALP and osteocalcin) and an increase of bone resorption markers (C-terminal telopeptides of collagen type 1 and tartrate-resistant acid phosphatase) were observed, but without statistical significance. This study aims to contribute to better knowledge of sheep as a model for osteoporosis research and the consequences that a performed induction protocol may impose on organic metabolism.

Original language	English
Article number	e20200435
Pages (from-to)	1-16
Number of pages	16
Journal	Anais da Academia Brasileira de Ciencias
Volume	92
Issue number	4
DOIs	https://doi.org/10.1590/0001-3765202020200435
Publication status	Published - 2020

Bibliographical note

Publisher Copyright:
© 2020, Academia Brasileira de Ciencias. All rights reserved.

Keywords

Animal model
Blood analysis
Bone marrow cytology
Lumbar vertebral micro-structure
Osteoporosis
Sheep

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10.1590/0001-3765202020200435

Cite this

Coelho, C. A., Bordelo, J. P., Camassa, J. A., Barros, V. A., Babo, P. S., Gomes, M. E., Reis, R. L., DE AZEVEDO, J. T., Requicha, J. F., Faísca, P., Carvalho, P. P., Viegas, C. A., & Dias, I. R. (2020). Evaluation of hematology, general serum biochemistry, bone turnover markers and bone marrow cytology in a glucocorticoid treated ovariectomized sheep model for osteoporosis research. Anais da Academia Brasileira de Ciencias, 92(4), 1-16. Article e20200435. https://doi.org/10.1590/0001-3765202020200435

@article{1313f56d749a401d82a08825aff3c109,

title = "Evaluation of hematology, general serum biochemistry, bone turnover markers and bone marrow cytology in a glucocorticoid treated ovariectomized sheep model for osteoporosis research",

abstract = "Osteoporosis is a metabolic disorder characterized by a loss of bone mass and structure and increasing the risk of fragility fractures, mostly among postmenopausal women. Sheep is a recognized large animal model for osteoporosis research. An experimental group of ewes (3-4 years old) was subjected to ovariectomy (OVX) and weekly glucocorticoid (GC) application for 24 weeks and compared with a sham control group. Blood and bone marrow parameters were analyzed before and 24 weeks after OVX and GC administration. Osteopenia was confirmed through micro-computed tomography and histomorphometric analysis of L4 vertebra in the study end. A statistically significant increase was observed in mean corpuscular volume, mean cell hemoglobin and monocytes and a decrease in red blood count and eosinophils (p<0.05). Alkaline phosphatase (ALP), gamma-glutamyl transpeptidase, magnesium and α1-globulin increased, and creatinine, albumin, sodium and estradiol decreased (p<0.05). A slight decrease of bone formation markers (bone ALP and osteocalcin) and an increase of bone resorption markers (C-terminal telopeptides of collagen type 1 and tartrate-resistant acid phosphatase) were observed, but without statistical significance. This study aims to contribute to better knowledge of sheep as a model for osteoporosis research and the consequences that a performed induction protocol may impose on organic metabolism.",

keywords = "Animal model, Blood analysis, Bone marrow cytology, Lumbar vertebral micro-structure, Osteoporosis, Sheep",

author = "Coelho, {Catarina A.} and Bordelo, {Jo{\~a}o P.} and Camassa, {Jos{\'e} A.} and Barros, {Vera A.} and Babo, {Pedro S.} and Gomes, {Manuela E.} and Reis, {Rui L.} and {DE AZEVEDO}, {Jorge T.} and Requicha, {Jo{\~a}o F.} and Pedro Fa{\'i}sca and Carvalho, {Pedro P.} and Viegas, {Carlos A.} and Dias, {Isabel R.}",

year = "2020",

doi = "10.1590/0001-3765202020200435",

language = "English",

volume = "92",

pages = "1--16",

journal = "Anais da Academia Brasileira de Ciencias",

issn = "0001-3765",

publisher = "Academia Brasileira de Ciencias",

number = "4",

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Coelho, CA, Bordelo, JP, Camassa, JA, Barros, VA, Babo, PS, Gomes, ME, Reis, RL, DE AZEVEDO, JT, Requicha, JF, Faísca, P, Carvalho, PP, Viegas, CA & Dias, IR 2020, 'Evaluation of hematology, general serum biochemistry, bone turnover markers and bone marrow cytology in a glucocorticoid treated ovariectomized sheep model for osteoporosis research', Anais da Academia Brasileira de Ciencias, vol. 92, no. 4, e20200435, pp. 1-16. https://doi.org/10.1590/0001-3765202020200435

Evaluation of hematology, general serum biochemistry, bone turnover markers and bone marrow cytology in a glucocorticoid treated ovariectomized sheep model for osteoporosis research. / Coelho, Catarina A.; Bordelo, João P.; Camassa, José A. et al.
In: Anais da Academia Brasileira de Ciencias, Vol. 92, No. 4, e20200435, 2020, p. 1-16.

Research output: Contribution to journal › Review article › peer-review

TY - JOUR

T1 - Evaluation of hematology, general serum biochemistry, bone turnover markers and bone marrow cytology in a glucocorticoid treated ovariectomized sheep model for osteoporosis research

AU - Coelho, Catarina A.

AU - Bordelo, João P.

AU - Camassa, José A.

AU - Barros, Vera A.

AU - Babo, Pedro S.

AU - Gomes, Manuela E.

AU - Reis, Rui L.

AU - DE AZEVEDO, Jorge T.

AU - Requicha, João F.

AU - Faísca, Pedro

AU - Carvalho, Pedro P.

AU - Viegas, Carlos A.

AU - Dias, Isabel R.

PY - 2020

Y1 - 2020

N2 - Osteoporosis is a metabolic disorder characterized by a loss of bone mass and structure and increasing the risk of fragility fractures, mostly among postmenopausal women. Sheep is a recognized large animal model for osteoporosis research. An experimental group of ewes (3-4 years old) was subjected to ovariectomy (OVX) and weekly glucocorticoid (GC) application for 24 weeks and compared with a sham control group. Blood and bone marrow parameters were analyzed before and 24 weeks after OVX and GC administration. Osteopenia was confirmed through micro-computed tomography and histomorphometric analysis of L4 vertebra in the study end. A statistically significant increase was observed in mean corpuscular volume, mean cell hemoglobin and monocytes and a decrease in red blood count and eosinophils (p<0.05). Alkaline phosphatase (ALP), gamma-glutamyl transpeptidase, magnesium and α1-globulin increased, and creatinine, albumin, sodium and estradiol decreased (p<0.05). A slight decrease of bone formation markers (bone ALP and osteocalcin) and an increase of bone resorption markers (C-terminal telopeptides of collagen type 1 and tartrate-resistant acid phosphatase) were observed, but without statistical significance. This study aims to contribute to better knowledge of sheep as a model for osteoporosis research and the consequences that a performed induction protocol may impose on organic metabolism.

AB - Osteoporosis is a metabolic disorder characterized by a loss of bone mass and structure and increasing the risk of fragility fractures, mostly among postmenopausal women. Sheep is a recognized large animal model for osteoporosis research. An experimental group of ewes (3-4 years old) was subjected to ovariectomy (OVX) and weekly glucocorticoid (GC) application for 24 weeks and compared with a sham control group. Blood and bone marrow parameters were analyzed before and 24 weeks after OVX and GC administration. Osteopenia was confirmed through micro-computed tomography and histomorphometric analysis of L4 vertebra in the study end. A statistically significant increase was observed in mean corpuscular volume, mean cell hemoglobin and monocytes and a decrease in red blood count and eosinophils (p<0.05). Alkaline phosphatase (ALP), gamma-glutamyl transpeptidase, magnesium and α1-globulin increased, and creatinine, albumin, sodium and estradiol decreased (p<0.05). A slight decrease of bone formation markers (bone ALP and osteocalcin) and an increase of bone resorption markers (C-terminal telopeptides of collagen type 1 and tartrate-resistant acid phosphatase) were observed, but without statistical significance. This study aims to contribute to better knowledge of sheep as a model for osteoporosis research and the consequences that a performed induction protocol may impose on organic metabolism.

KW - Animal model

KW - Blood analysis

KW - Bone marrow cytology

KW - Lumbar vertebral micro-structure

KW - Osteoporosis

KW - Sheep

UR - http://www.scopus.com/inward/record.url?scp=85097517797&partnerID=8YFLogxK

U2 - 10.1590/0001-3765202020200435

DO - 10.1590/0001-3765202020200435

M3 - Review article

C2 - 33295580

AN - SCOPUS:85097517797

SN - 0001-3765

VL - 92

SP - 1

EP - 16

JO - Anais da Academia Brasileira de Ciencias

JF - Anais da Academia Brasileira de Ciencias

IS - 4

M1 - e20200435

ER -

Evaluation of hematology, general serum biochemistry, bone turnover markers and bone marrow cytology in a glucocorticoid treated ovariectomized sheep model for osteoporosis research

Abstract

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Keywords

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