Abstract
The degradation of alkanes and alcohols by Rhodococcus erythropolis DCL14 growing cells was tested at different reactor scales, ranging from a 2-L fermenter to 300 μL 96-well plates. The highest growth rates were attained in the fermenter, where pH, temperature and aeration rate, in particular, are monitored and controlled. However, the results acquired in reactors smaller than 100 mL indicate that these reactors may be successfully used in testing carbon sources, since the normalized results obtained with the different carbons sources were maintained in the 100-0.3 mL range. This study also shows the great potential of R. erythropolis DCL14 cells to degrade a wide range of alcohols and alkanes, which may be used in bioremediation of contaminated sites.
Original language | English |
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Pages (from-to) | 144-150 |
Number of pages | 7 |
Journal | Biocatalysis and Biotransformation |
Volume | 25 |
Issue number | 2-4 |
DOIs | |
Publication status | Published - Mar 2007 |
Funding
This work was supported by two post-doctoral grants, SFRH/BPD/14426/2003 and SFRH/BPD/ 20416/2004, awarded to Carla C. C. R. de Carvalho and Pedro Fernandes, respectively, and by a PhD grant (SFRH/BD/24433/2005) awarded to Marco P. C. Marques by Fundac¸ão para a Ciência e a Tecnologia, Portugal.
Funders | Funder number |
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Fundac¸ão para a Ciência e a Tecnologia |
Keywords
- Bacteria
- Biodegradation
- Bioreactor
- Bioremediation
- Membrane composition