TY - JOUR
T1 - Characterization of gastric cells infection by diverse Helicobacter pylori strains through Fourier-transform infrared spectroscopy
AU - Marques, Vanda
AU - Cunha, Bernardo
AU - Couto, Andreia
AU - Sampaio, Pedro
AU - Fonseca, Luís P.
AU - Aleixo, Sandra
AU - Calado, Cecília R.C.
N1 - Publisher Copyright:
© 2018 Elsevier B.V.
PY - 2019/3/5
Y1 - 2019/3/5
N2 - The infection of Helicobacter pylori, covering 50% of the world-population, leads to diverse gastric diseases as ulcers and cancer along the life-time of the human host. To promote the discovery of biomarkers of bacterial infection, in the present work, Fourier-transform infrared spectra were acquired from adenocarcinoma gastric cells, incubated with H. pylori strains presenting different genotypes concerning the virulent factors cytotoxin associated gene A and vacuolating cytotoxin A. Defined absorbance ratios were evaluated by diverse methods of statistical inference, according to the fulfillment of the tests assumptions. It was possible to define from the gastric cells, diverse absorbance ratios enabling to discriminate: i) The infection; ii) the bacteria genotype; and iii) the gastric disease of the patients from which the bacteria were isolated. These biomarkers could fasten the knowledge of the complex infection process while promoting a platform for a new diagnostic method, rapid but also specific and sensitive towards the diagnosis of both infection and bacterial virulence.
AB - The infection of Helicobacter pylori, covering 50% of the world-population, leads to diverse gastric diseases as ulcers and cancer along the life-time of the human host. To promote the discovery of biomarkers of bacterial infection, in the present work, Fourier-transform infrared spectra were acquired from adenocarcinoma gastric cells, incubated with H. pylori strains presenting different genotypes concerning the virulent factors cytotoxin associated gene A and vacuolating cytotoxin A. Defined absorbance ratios were evaluated by diverse methods of statistical inference, according to the fulfillment of the tests assumptions. It was possible to define from the gastric cells, diverse absorbance ratios enabling to discriminate: i) The infection; ii) the bacteria genotype; and iii) the gastric disease of the patients from which the bacteria were isolated. These biomarkers could fasten the knowledge of the complex infection process while promoting a platform for a new diagnostic method, rapid but also specific and sensitive towards the diagnosis of both infection and bacterial virulence.
KW - Biomarkers
KW - Gastric adenocarcinoma cells
KW - Helicobacter pylori
KW - Infrared spectroscopy
UR - http://www.scopus.com/inward/record.url?scp=85056641406&partnerID=8YFLogxK
U2 - 10.1016/j.saa.2018.11.001
DO - 10.1016/j.saa.2018.11.001
M3 - Article
C2 - 30453195
AN - SCOPUS:85056641406
SN - 1386-1425
VL - 210
SP - 193
EP - 202
JO - Spectrochimica Acta - Part A: Molecular and Biomolecular Spectroscopy
JF - Spectrochimica Acta - Part A: Molecular and Biomolecular Spectroscopy
ER -