Characterization of gastric cells infection by diverse Helicobacter pylori strains through Fourier-transform infrared spectroscopy

Vanda Marques, Bernardo Cunha, Andreia Couto, Pedro Sampaio, Luís P. Fonseca, Sandra Aleixo, Cecília R.C. Calado

Research output: Contribution to journalArticlepeer-review

24 Citations (Scopus)

Abstract

The infection of Helicobacter pylori, covering 50% of the world-population, leads to diverse gastric diseases as ulcers and cancer along the life-time of the human host. To promote the discovery of biomarkers of bacterial infection, in the present work, Fourier-transform infrared spectra were acquired from adenocarcinoma gastric cells, incubated with H. pylori strains presenting different genotypes concerning the virulent factors cytotoxin associated gene A and vacuolating cytotoxin A. Defined absorbance ratios were evaluated by diverse methods of statistical inference, according to the fulfillment of the tests assumptions. It was possible to define from the gastric cells, diverse absorbance ratios enabling to discriminate: i) The infection; ii) the bacteria genotype; and iii) the gastric disease of the patients from which the bacteria were isolated. These biomarkers could fasten the knowledge of the complex infection process while promoting a platform for a new diagnostic method, rapid but also specific and sensitive towards the diagnosis of both infection and bacterial virulence.

Original languageEnglish
Pages (from-to)193-202
Number of pages10
JournalSpectrochimica Acta - Part A: Molecular and Biomolecular Spectroscopy
Volume210
DOIs
Publication statusPublished - 5 Mar 2019

Bibliographical note

Publisher Copyright:
© 2018 Elsevier B.V.

Keywords

  • Biomarkers
  • Gastric adenocarcinoma cells
  • Helicobacter pylori
  • Infrared spectroscopy

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