Biocatalysis of steroids with Mycobacterium sp. In aqueous and organic media

Carla C.C.R. de Carvalho, Pedro Fernandes

Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review

1 Citation (Scopus)

Abstract

Mycobacterium sp. can convert steroids such as β-sitosterol, campesterol, and cholesterol into commercially interesting products. In aqueous systems, the biocatalysis is limited by the low solubility of the steroids in water. This may be overcome by the introduction of an organic phase acting as a substrate and/or product reservoir. In this chapter, we describe the biocatalysis of β-sitosterol to 4-androstene-3,17-dione (AD) and 1,4-androstadiene-3,17-dione (ADD) both in aqueous and organic-aqueous systems. In the latter case, both traditional organic solvents and green solvents are proposed.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages313-320
Number of pages8
DOIs
Publication statusPublished - 2017

Publication series

NameMethods in Molecular Biology
Volume1645
ISSN (Print)1064-3745

Bibliographical note

Publisher Copyright:
© Springer Science+Business Media LLC 2017.

Keywords

  • Bioconversion
  • Biotransformation
  • Cholesterol
  • Green solvents
  • Organic solvents
  • Two-phase systems
  • β-Sitosterol

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