A simple imaging method for biomass determination

Carla C.C.R. De Carvalho; Marco P.C. Marques; Pedro Fernandes; M. Manuela R. Da Fonseca

doi:10.1016/j.mimet.2004.09.014

A simple imaging method for biomass determination

Carla C.C.R. De Carvalho, Marco P.C. Marques, Pedro Fernandes, M. Manuela R. Da Fonseca

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8 Citations (Scopus)

Abstract

An inexpensive and fast method based on images taken during growth of bacterial cells on multi-well plates was developed for biomass quantification. A correlation of 85% between the results obtained by image analysis and optical density measurements was obtained. This simple method allows the assessment of growth with highly aggregated cell cultures and the rapid screening of a large number of carbon sources.

Original language	English
Pages (from-to)	135-140
Number of pages	6
Journal	Journal of Microbiological Methods
Volume	60
Issue number	1
DOIs	https://doi.org/10.1016/j.mimet.2004.09.014
Publication status	Published - Jan 2005

Funding

This study was supported by a post-doctoral grant (SFRH/BPD/14426/2003) awarded to Carla da C.C.R. de Carvalho by Fundação para a Ciência e a Tecnologia, Portugal.

Funders	Funder number
Fundação para a Ciência e a Tecnologia

Keywords

Cell clustering
Growth quantification
Image analysis
Substrate screening
Toxic substrates

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10.1016/j.mimet.2004.09.014

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title = "A simple imaging method for biomass determination",

abstract = "An inexpensive and fast method based on images taken during growth of bacterial cells on multi-well plates was developed for biomass quantification. A correlation of 85% between the results obtained by image analysis and optical density measurements was obtained. This simple method allows the assessment of growth with highly aggregated cell cultures and the rapid screening of a large number of carbon sources.",

keywords = "Cell clustering, Growth quantification, Image analysis, Substrate screening, Toxic substrates",

author = "{De Carvalho}, {Carla C.C.R.} and Marques, {Marco P.C.} and Pedro Fernandes and {Da Fonseca}, {M. Manuela R.}",

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AU - Fernandes, Pedro

AU - Da Fonseca, M. Manuela R.

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KW - Growth quantification

KW - Image analysis

KW - Substrate screening

KW - Toxic substrates

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A simple imaging method for biomass determination

Abstract

Funding

Keywords

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