β-sitosterol bioconversion to androstenedione in microtiter plates

Marco P.C. Marques; Pedro Fernandes

doi:10.1007/978-1-4939-7183-1_12

β-sitosterol bioconversion to androstenedione in microtiter plates

Marco P.C. Marques, Pedro Fernandes

Research output: Chapter in Book/Report/Conference proceeding › Chapter › peer-review

4 Citations (Scopus)

Abstract

Microtiter plates are routinely used as low-cost miniaturized bioreactors due to the large number of experiments that can be conducted simultaneously under similar conditions and replicate all functions of bench-scale reactors at dramatically smaller volumes. These plates, due to the standard footprint, can be integrated with liquid-handling systems and associated equipment expanding considerably their application and use. However, care has to be taken to operate the microtiter plates in optimized mixing and oxygen transfer conditions, preventing medium evaporation in prolonged experiment runs. Here, we describe the production of 4-androstene-3,17-dione (androstenedione; AD), a key pharmaceutical steroid intermediate, by Mycobacterium sp. NRRL B-3805 via the selective cleavage of the side-chain of β-sitosterol using 24-well microtiter plates.

Original language	English
Title of host publication	Methods in Molecular Biology
Publisher	Humana Press Inc.
Pages	167-176
Number of pages	10
DOIs	https://doi.org/10.1007/978-1-4939-7183-1_12
Publication status	Published - 2017

Publication series

Name	Methods in Molecular Biology
Volume	1645
ISSN (Print)	1064-3745

Bibliographical note

Publisher Copyright:
© Springer Science+Business Media LLC 2017.

Keywords

4-Androstene-3,17-dione
AD
Evaporation
High-throughput screening
Microtiter plate
Mycobacterium sp. NRRL B-3805
Steroid
β-Sitosterol

Access to Document

10.1007/978-1-4939-7183-1_12

Cite this

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abstract = "Microtiter plates are routinely used as low-cost miniaturized bioreactors due to the large number of experiments that can be conducted simultaneously under similar conditions and replicate all functions of bench-scale reactors at dramatically smaller volumes. These plates, due to the standard footprint, can be integrated with liquid-handling systems and associated equipment expanding considerably their application and use. However, care has to be taken to operate the microtiter plates in optimized mixing and oxygen transfer conditions, preventing medium evaporation in prolonged experiment runs. Here, we describe the production of 4-androstene-3,17-dione (androstenedione; AD), a key pharmaceutical steroid intermediate, by Mycobacterium sp. NRRL B-3805 via the selective cleavage of the side-chain of β-sitosterol using 24-well microtiter plates.",

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AU - Fernandes, Pedro

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KW - High-throughput screening

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KW - Mycobacterium sp. NRRL B-3805

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β-sitosterol bioconversion to androstenedione in microtiter plates

Abstract

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Bibliographical note

Keywords

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